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The measurement volume was calibrated on each experimental day with 20 nM Rhodamine 6 G (Diffusion Coefficient, D, 2.8 x10 -10 m 2 s -1 ) in HPLC grade water
None of this is administration guidance
Cells were then washed with KRBH and imaged in 3 mM glucose KRBH with images taken every 12 seconds in a 60/1.4 NA oil immersion objective with a 561 nm laser in a Nikon Eclipse Ti microscope with an ORCA-Flash 4.0 camera (Hamamatsu) and Metamorph software (Molecular Devices)
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The most common adverse reactions of higher doses from 15 to 30 grams daily are nausea, abdominal cramps, and diarrhea
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