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Compared to sgRNA controls, sg CBS significantly reduced intracellular cystathionine, cysteine, and GSH levels

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And then the membrane was incubated with mouse monoclonal anti-hexahistidine monoclonal antibody (Sigma) diluted (1:3000) in TTBS at 37C for 1 h

[DOI] [PubMed] [Google Scholar] 47.Ferraz A.C., da Silva Menegatto M.B., Lima R.L.S., Ola-Olub O.S., Costa D.C., de Magalhes J.C., Rezende I.M., LaBeaud A.D., Monath T.P., Alves P.A., et al