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Most people vape between 100-500 puffs a day, but it can vary widely based on: device nicotine strength puff length personal habits The best approach to nicotine control is to monitor puff counts, be aware of your nicotine intake, and make vaping work for you as a tool to quit or reduce cigarette smoking

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Cultures were dislodged from the insert with 200 L apical dissociation solution (Accutase with 5 mM EDTA and 5 mM EGTA) for 30 min at 37 C with occasional manual agitation 68