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In fact, it calls to mind Allens arguably best-known work, which would come years later: Gritty, the beloved orange, googly-eyed, unhinged mascot of the Philadelphia Flyers

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As our in vivo scRNA-seq data did not wholly capture the heterogeneity reflective of this progression, we studied the process by culturing basal tracheal epithelial cells at airliquid interface (ALI) and harvesting replicate cultures at 20 timepoints across mucociliary differentiation for scRNA-seq analysis (Fig